Genotypic Heterogeneity Precise Microdissection of Human Prostate Cancers Reveals
نویسندگان
چکیده
To determine the incidence of genetic heterogeneity in primary prostate cancer, we have microdissected 125 tumor and mesenchymal foci from 18 patient biopsies and analyzed the DNA for loss of het erozygosity using PCR microsatellite markers. In 100% of patients with genetic lesions on chromosome Sp, there was evidence for intra tumoral genetic heterogeneity. There was also a low but significant Incidence of loss of heterozygosity in mesenchymal tissue. Our results show that phenotypically similar tumor foci can have different geno types and provide evidence for the multifocality of tumor development In the prostate.
منابع مشابه
Precise microdissection of human prostate cancers reveals genotypic heterogeneity.
To determine the incidence of genetic heterogeneity in primary prostate cancer, we have microdissected 125 tumor and mesenchymal foci from 18 patient biopsies and analyzed the DNA for loss of heterozygosity using PCR microsatellite markers. In 100% of patients with genetic lesions on chromosome 8p, there was evidence for intratumoral genetic heterogeneity. There was also a low but significant i...
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